Baltic Summer University course: Methods in Molecular Genetics
This course is a comprehensive overview of key techniques in molecular biology mostly related to DNA analysis. The course covers the techniques of DNA/RNA extraction, gel electrophoresis, PCR and restriction digestion; data analysis using computer software. Upon completion of this course, the trainee would be able to extract genomic DNA/RNA from animal cells, perform agarose gel electrophoresis to analyse the DNA profile; amplify specific fragments of DNA using the polymerase chain reaction (PCR) technique; perform restriction digestion experiments and visualise data obtained. School consists of lectures, practical exercises in laboratories, data analysis and case studies seminars.
Topics:
- basic genetics knowledge
- DNA extraction
- gel electrophoresis
- polymerase chain reaction
- restriction digestion
- data visualisation and interpretation